• Design Fusion Protein to facilitate GPCR cryo-EM study with enhanced stability and rigidity
• Use RFdiffusion + AF2 workflow. Delete the third intracellular loop (ICL3) and generate 10-20 amino acids between ICL3 and TM5/TM6. After forming a stable helix, extend the backbone generation to 100-150 amino acids. Use ProteinMPNN for side chain packing